Journal: European Journal of Pharmaceutical Sciences
Article Title: Model-based global sensitivity analysis as applied to identification of anti-cancer drug targets and biomarkers of drug resistance in the ErbB2/3 network
doi: 10.1016/j.ejps.2011.10.026
Figure Lengend Snippet: Experimental testing of GSA-derived combination therapies. Integrated pAkt response to heregulin-β stimulation in PE04 (left) and OVCAR4 (right) cell lines, treated with combinations of pertuzumab, LY294002 (LY) and UCN-01 (UCN). The data were normalised on the AUC of pAkt time-course in the absence of any drugs.
Article Snippet: Cells were treated with UCN-01 (protein kinase inhibitor; Calbiochem #539644; final concentration of 1 μM), LY294002 (PI3 kinase inhibitor; Calbiochem #440204; final concentration 20 μM), Pertuzumab (ErbB2 inhibitor; final concentration 100 nM) and stimulation by Heregulin (R&D Systems; 396-HB-CF) was at final concentration of 1 nM.
Techniques: Derivative Assay